Nuclear Transfer in Bovine Embryos
نویسندگان
چکیده
منابع مشابه
Cellular evaluation of bovine nuclear transfer embryos developed in vitro.
Cloned blastocysts developed in vitro for 7 d had a mean number of cells (82.86 +/- 5.35) as evaluated by nuclei counting in serial optical sections using confocal microscopy, after staining with propidium iodide. This number was not significantly different from that of control IVF embryos cultured under the same conditions during the same period (mean = 88.89 +/- 7.53). Semi-thin sections reve...
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This study was performed to examine whether adult fibroblast cells can be manipulated genetically and whether clonal lines derived from those cells can support embryonic development. A primary adult cell line was established from the ear of an aged cow. Adult cells with a plasmid containing the enhancer green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning. Gre...
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This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic cells were fused to enucleated oocytes treated with either 5 mM caffeine or 150 microM roscovitine to control the type of nuclear remodeling. The proportion of embryos that under...
متن کاملReprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos
BACKGROUND Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive. METHODOLOGY/PRINCIPAL FINDINGS In this study, we analyzed the EGA-related transcriptome of rhesus-bovine iSCNT 8- to 16-cell embryos and dissected the reprogra...
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To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage based on the traditional culture method, and analyzed the expression level of the genes related to blastocyst hatching (Na/K-ATPase, Glut-1) and related to activin A signalling pathway (Act...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1997
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.97-436j113